---
title: Download - Cell disruption of S. cerevisiae by scalable high-intensity ultrasound
description: Directly scalable ultrasonic cell disruption (lysing) technology is based on large-diameter Barbell horns that provide high cavitation intensities.
---

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# Cell Disruption of S. cerevisiae by Scalable High-Intensity Ultrasound

- **Gives background on ultrasonic cell disruption (lysing) as the most effective laboratory method for the recovery of recombinant proteins;**
- **Explains why with conventional sonication equipment, ultrasonic cell disruption has only been feasible on a laboratory scale;**
- **Shows how with [Barbell Horn](https://www.sonomechanics.com/barbell-horn-ultrasonic-technology/)® Ultrasonic Technology ultrasonic cell disruption can be successfully scaled up and used in industrial production.**

*Simon Bystryak, Alexey S. Peshkovsky, Rasa Santockytea. Chemical Engineering and Processing: Process Intensification,  Volume 99, July 2015, pages 99-106.*

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Ultrasonic disruption of yeast and other microbial cell cultures is commonly used for laboratory-scale protein preparations because it is rapid, efficient and simple to use compared to such methods as high-pressure homogenization (HPH) and bead milling. Lysing by sonication is also more effective than other cell disruption methods for the recovery of periplasmic, membrane-bound, or insoluble recombinant  
proteins. Until recently, however, due to amplitude limitations of conventional-design pilot and industrial-size sonication equipment, ultrasonic cell disruption was only feasible on the laboratory scale. In this study, we show that Barbell Horn Ultrasonic Technology ([**BHUT**](https://www.sonomechanics.com/barbell-horn-ultrasonic-technology/)) can be successfully used for the disruption of Sacharomyces cerevisiae (S. cerevisiae) cells on a large scale. In particular, we show that  
by using pilot-scale BHUT-based equipment, total protein and alkaline phosphatase can be efficiently extracted from S. cerevisiae cells, achieving about an order of magnitude productivity increase factor with respect to laboratory-scale results. Since the size of BHUT-based ultrasonic processors can be increased  
further, ultrasonic cell disruption now has the ability to develop into a valuable commercial-scale method, potentially superior to HPH and bead milling techniques in this area of application.

#### About Us

Industrial Sonomechanics is a R&D, equipment design and process consulting firm, specializing in high-power ultrasonic technology for liquid treatment. Our mission is to help businesses implement their ultrasound-assisted processes on lab, pilot and industrial scales.

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